V-ATPase | epsilon subunit of tonoplast H+ATPase, Blocking peptide
Estimation of the ratio between antibodies and a peptide : In neutralisation assay use 8,13 l of V-ATPase peptide for 10 ml of reaction volume. In case of any questions please contact support@agrisera
AgriCoat-Block | Non mammalian Protein Coat Block (5X)
Recommendation for use:Can be used for antigens and antibody coating, blocking or as a diluent. Blocking and stabilization in one step may allow to eliminated an additional blocking step. Need to be e
Rat IgG, Purified, (1 gram)
Purity is > 95% based on SDS-PAGE.Supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 0.05% (w/v).For use as a standard or control in most immunoassay formats.
Donkey normal serum (2 ml)
Recommended dilution: 2-5% (v/v) serum in PBS/TBS. To make a 2% (v/v) solution: add 2 ml of Normal Donkey Serum to 98 ml of PBS/TBS. Also, adding 0.05% Tween-20 will be beneficial.For a Western Blot