Tubulin alpha and gamma are not suitable as a loading control for metal stress-分析方法-资讯-生物在线

Tubulin alpha and gamma are not suitable as a loading control for metal stress

作者:Agrisera AB 2021-04-12T00:00 (访问量:2314)

Can tubulin be used as a loading control in metal stress?

To check this  Anti-tubulin alpha chain antibodies were used on extracts from:

Nicotiana tabacum plants were treated with silver nanoparticles (AgNPs) with 2 different coatings.

Tubulin alpha protein content marked by a blue arrow in the image to the right, was influenced by silver nanoparticle treatment. Similar results were obtained for tubulin gamma chain

Therefore, it can be concluded that:

Metal induced stress affected the expression of tubulin alpha and gamma, and that therefore, these proteins cannot be used as loading control under these conditions.




 
Western blot using anti-tubulin antibodies on metal stressed samples
All experimental details can be found here.
Courtesy of MSc, Karla Košpić, University of Zagreb, Faculty of Science Department of Biology, Croatia
Agrisera AB 商家主页

地 址: 21 Vännäs, SWEDEN, Europe

联系人: Dr. Joanna Porankiewicz-Asplund

电 话: 0046704137107

传 真:

Email:61382170@qq.com

相关咨询
ADVERTISEMENT